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Isolation and Identification of Local Ethanol Tolerant Yeast Populating Distillation and Milling Sites in Nigeria

Received: 25 April 2016     Accepted: 25 August 2016     Published: 12 September 2016
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Abstract

Two yeast strains referred to as OY and SY were isolated and characterised from local distillery and cereal milling sites. Isolation was done using potato dextrose media supplemented with 0.2% chloramphenicol. Morphological and biochemical results together with the rDNA internal transcribed spacer region (ITS) were identified as Pichia kudriavzevii strains GY1 and L9 respectively. OY and SY are ethanol tolerant strains, withstanding ethanol concentrations of up to 20% (v/v) in yeast extract, peptone, malt extract, glucose media. OY and SY displayed good growth in ethanol supplemented medium with pH ranging from 4.8-5.5 at 30°C. Growth measurements were determined by measuring optical density of the cells in broth using spectrophotometer at 570nm. The results obtained suggested that OY and SY demonstrated good parameters as ideal candidates for bioethanol production.

Published in American Journal of BioScience (Volume 4, Issue 5)
DOI 10.11648/j.ajbio.20160405.11
Page(s) 58-63
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Ethanol, Ethanol Tolerance, Saccharomyces, Non-saccharomyces, Pichia kudriavzevii

References
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Cite This Article
  • APA Style

    Gidado Rose Suniso Maxwell, Etim Veronica Archibong, Nweke Ogechi, Iloh Andrew Chibuzor, Isu Rosemary Nennaya, et al. (2016). Isolation and Identification of Local Ethanol Tolerant Yeast Populating Distillation and Milling Sites in Nigeria. American Journal of BioScience, 4(5), 58-63. https://doi.org/10.11648/j.ajbio.20160405.11

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    ACS Style

    Gidado Rose Suniso Maxwell; Etim Veronica Archibong; Nweke Ogechi; Iloh Andrew Chibuzor; Isu Rosemary Nennaya, et al. Isolation and Identification of Local Ethanol Tolerant Yeast Populating Distillation and Milling Sites in Nigeria. Am. J. BioScience 2016, 4(5), 58-63. doi: 10.11648/j.ajbio.20160405.11

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    AMA Style

    Gidado Rose Suniso Maxwell, Etim Veronica Archibong, Nweke Ogechi, Iloh Andrew Chibuzor, Isu Rosemary Nennaya, et al. Isolation and Identification of Local Ethanol Tolerant Yeast Populating Distillation and Milling Sites in Nigeria. Am J BioScience. 2016;4(5):58-63. doi: 10.11648/j.ajbio.20160405.11

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  • @article{10.11648/j.ajbio.20160405.11,
      author = {Gidado Rose Suniso Maxwell and Etim Veronica Archibong and Nweke Ogechi and Iloh Andrew Chibuzor and Isu Rosemary Nennaya and Solomon Bamidele Ogbe},
      title = {Isolation and Identification of Local Ethanol Tolerant Yeast Populating Distillation and Milling Sites in Nigeria},
      journal = {American Journal of BioScience},
      volume = {4},
      number = {5},
      pages = {58-63},
      doi = {10.11648/j.ajbio.20160405.11},
      url = {https://doi.org/10.11648/j.ajbio.20160405.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20160405.11},
      abstract = {Two yeast strains referred to as OY and SY were isolated and characterised from local distillery and cereal milling sites. Isolation was done using potato dextrose media supplemented with 0.2% chloramphenicol. Morphological and biochemical results together with the rDNA internal transcribed spacer region (ITS) were identified as Pichia kudriavzevii strains GY1 and L9 respectively. OY and SY are ethanol tolerant strains, withstanding ethanol concentrations of up to 20% (v/v) in yeast extract, peptone, malt extract, glucose media. OY and SY displayed good growth in ethanol supplemented medium with pH ranging from 4.8-5.5 at 30°C. Growth measurements were determined by measuring optical density of the cells in broth using spectrophotometer at 570nm. The results obtained suggested that OY and SY demonstrated good parameters as ideal candidates for bioethanol production.},
     year = {2016}
    }
    

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    AU  - Gidado Rose Suniso Maxwell
    AU  - Etim Veronica Archibong
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    AU  - Iloh Andrew Chibuzor
    AU  - Isu Rosemary Nennaya
    AU  - Solomon Bamidele Ogbe
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    N1  - https://doi.org/10.11648/j.ajbio.20160405.11
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    T2  - American Journal of BioScience
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    SN  - 2330-0167
    UR  - https://doi.org/10.11648/j.ajbio.20160405.11
    AB  - Two yeast strains referred to as OY and SY were isolated and characterised from local distillery and cereal milling sites. Isolation was done using potato dextrose media supplemented with 0.2% chloramphenicol. Morphological and biochemical results together with the rDNA internal transcribed spacer region (ITS) were identified as Pichia kudriavzevii strains GY1 and L9 respectively. OY and SY are ethanol tolerant strains, withstanding ethanol concentrations of up to 20% (v/v) in yeast extract, peptone, malt extract, glucose media. OY and SY displayed good growth in ethanol supplemented medium with pH ranging from 4.8-5.5 at 30°C. Growth measurements were determined by measuring optical density of the cells in broth using spectrophotometer at 570nm. The results obtained suggested that OY and SY demonstrated good parameters as ideal candidates for bioethanol production.
    VL  - 4
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Author Information
  • Agricultural Biotechnology Department, National Biotechnology Development Agency (NABDA), Abuja, Nigeria

  • Biotechnology Advanced Research Center, Sheda Science and Technology Complex (SHESTCO), Abuja, Nigeria

  • Biotechnology Advanced Research Center, Sheda Science and Technology Complex (SHESTCO), Abuja, Nigeria

  • Biotechnology Advanced Research Center, Sheda Science and Technology Complex (SHESTCO), Abuja, Nigeria

  • Department of Biological Sciences, University of Abuja, Abuja, Nigeria

  • Department of Chemical Engineering, Obafemi Awolowo University, Ile-Ife, Nigeria

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